¹⁴C-Fluorodeoxyglucose-autoradiography:

coronal section through the forebrain of an acoustically imprinted chick during exposure to the acoustic imprinting stimulus.

Immunohistochemistry:

in vitro:

double-Immuno staining,

rat, primary culture (14 days).

confocal laserscan microscopy:

filled LY-pyramidal cell, 3D-reconstruction

(Octodon degus).

► Immunohistochemistry

►¹⁴C-Fluorodeoxyglucose-autoradiography (analysis of stimulus evoked metabolic brain activity)

►³H receptor-autoradiography: ligand binding for transmitter-peptide and hormone receptors.

► quantitative light microscopy, confocal laserscan microscopy, e.g. immunocytochemical detection and ultrastructural localization of transmitter receptors, transmitter, Ca-binding proteins, synapse-specific proteins, enzymes

► quantitative electron microscopy

► in situ hybridization techniques

► iontophoretic and pressure injection of fluorescent dyes in living and fixed neurons for three dimensional reconstruction and quantitative morphological analysis of dendritic trees and dendritic spines

► computer-assisted image analysis for densitometry of autoradiographs, 3D-reconstruction of neurons (measurement of soma size, dendritic ramification patterns, density and spatial arrangement of dendritic spines), 3D-measurements of spine morphology (analysis system developed in our lab) and quantification of ultrastructural synaptic parameters

► pharmacological behavioral designs, behavioral experiments for emotional behavior, learning and memory formation

► in vivo microdialysis: quantitative measurements of amino acids, monoamines and their metabolites in freely behaving animals

► cell cultures: organotypic cocultures of forebrain and thalamus, primary cultures of hippocampal and cortical neurons

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